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Image Search Results
Journal: bioRxiv
Article Title: C. elegans synMuv B proteins regulate spatial and temporal chromatin compaction during development
doi: 10.1101/538801
Figure Lengend Snippet: (A) A cartoon representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. (B) Large extrachromosomal arrays containing numerous lacO sites represents a pseudo chromosome that when bound by LacI::GFP is used to visualize chromatin morphology. Intestinal nuclei were scored as open or closed based on array morphology . L4 hermaphrodites were placed at 20°C or 26°C and mixed stage F1 embryos were collected and percentage of intestinal nuclei with open arrays at the 8E, 16E, comma, and L1 stage at 20°C and 26°C were scored. 2-way ANOVA was used to determine significance: Asterisks represent significant difference between the wild-type population at that stage and temperature (p<0.01). Error bars indicate standard error of the proportion. n>100 cells (C) 3-D volumetric chromatin compaction analysis was performed using the isosurface function in Metamorph. The volume of the nucleus and the array were measured and used to calculate the percentage of the nucleus that the array encompassed. The percentage of the nucleus contained by the array was plotted at 8E, 16E, and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (2-way Anova p<0.01).
Article Snippet: Images were captured on Nikon Confocal and analyzed using
Techniques:
Journal: Development (Cambridge, England)
Article Title: C. elegans synMuv B proteins regulate spatial and temporal chromatin compaction during development
doi: 10.1242/dev.174383
Figure Lengend Snippet: Loss of synMuv B proteins causes a delay in chromatin compaction in the intestinal lineage during development. (A) Schematic representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. EMS, the precursor cell to the E lineage. (B) Representative images of intestinal nuclei with open and closed extra-chromosomal arrays. In merged image, green is LacI::GFP and red is elt-2p::dTomato. (C) Intestinal nuclei were scored as open or closed based on array morphology (Yuzyuk et al., 2009) in worms raised at 20°C and 26°C at the 8E, 16E, comma and L1 stages. Asterisks represent significant difference between the wild-type population at that stage and temperature (P<0.01, two-way ANOVA). Error bars indicate s.e.m. (D) Representative masked images of intestinal cell nuclei with open or closed extra-chromosomal arrays using the isosurface function in Metamorph; green is LacI::GFP and red is elt-2p::dTomato. (E) The array volume to nuclear volume ratio was measured using the isosurface function in Metamorph at 8E, 16E and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (P<0.01, two-way ANOVA).
Article Snippet: Error bars indicate s.e.m. (D) Representative masked images of intestinal cell nuclei with open or closed extra-chromosomal arrays using the
Techniques: